Vergleich

Anti-INPPL1 Antibody Picoband™

Hersteller Boster
Kategorie
Typ Antibody Primary
Specific against Human, Mouse, Rat
Isotype IgG
Format Lyophilized
Applikationen WB, FC, IF, IHC, ICC, ELISA
Menge 100 ug/vial
Host Rabbit
ArtNr BOS-A01790
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Manufacturers Product Category
Primary Antibodies, Rabbit Polyclonal Antibodies
Manufacturers Research Category
Actin Assembly, Actin, etc., Angiogenesis, Autoimmune, Cancer, Cardiovascular, Cytoskeleton, Cytoskeleton/ECM, Growth Factors, Immune System Diseases, Immunology, Lipid Signaling, Metabolism, Microfilaments, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction, Signaling Pathway
Short Description
Boster Bio Anti-INPPL1 Antibody Picoband™ catalog # A01790. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Description
Boster Bio Anti-INPPL1 Antibody Picoband™ catalog # A01790. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Background
SH2-domain containing Phosphatidylinositol-3, 4, 5-trisphosphate 5-phosphatase 2 is an enzyme that in humans is encoded by the INPPL1 gene. The protein encoded by this gene is an SH2-containing 5'-inositol phosphatase that is involved in the regulation of insulin function. The encoded protein also plays a role in the regulation of epidermal growth factor receptor turnover and actin remodelling. Additionally, this gene supports metastatic growth in breast cancer and is a valuable biomarker for breast cancer.
Gene Name
INPPL1
Application Details
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml
Application Notes
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Clonality
Polyclonal
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen
E. coli-derived human INPPL1 recombinant protein (Position: R1172-K1258).
Cross-reactivity
No cross-reactivity with other proteins.
Storage
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Gene Full Name
inositol polyphosphate phosphatase-like 1
Molecular Weight
150 kDa
Protein Function
Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol- 3, 4, 5-trisphosphate (PtdIns (3, 4, 5)P3) to produce PtdIns (3, 4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Plays a central role in regulation of PI3K-dependent insulin signaling, although the precise molecular mechanisms and signaling pathways remain unclear. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signaling or GLUT4 trafficking. Confers resistance to dietary obesity. May act by regulating AKT2, but not AKT1, phosphorylation at the plasma membrane. Part of a signaling pathway that regulates actin cytoskeleton remodeling. Required for the maintenance and dynamic remodeling of actin structures as well as in endocytosis, having a major impact on ligand-induced EGFR internalization and degradation. Participates in regulation of cortical and submembraneous actin by hydrolyzing PtdIns (3, 4, 5)P3 thereby regulating membrane ruffling (PubMed:21624956). Regulates cell adhesion and cell spreading. Required for HGF-mediated lamellipodium formation, cell scattering and spreading. Acts as a negative regulator of EPHA2 receptor endocytosis by inhibiting via PI3K-dependent Rac1 activation. Acts as a regulator of neuritogenesis by regulating PtdIns (3, 4, 5)P3 level and is required to form an initial protrusive pattern, and later, maintain proper neurite outgrowth. Acts as a negative regulator of the FC-gamma- RIIA receptor (FCGR2A). Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Involved in EGF signaling pathway. Upon stimulation by EGF, it is recruited by EGFR and dephosphorylates PtdIns (3, 4, 5)P3. Plays a negative role in regulating the PI3K-PKB pathway, possibly by inhibiting PKB activity. Down-regulates Fc-gamma-R-mediated phagocytosis in macrophages independently of INPP5D/SHIP1. In macrophages, down-regulates NF-kappa-B-dependent gene transcription by regulating macrophage colony-stimulating factor (M-CSF)-induced signaling. May also hydrolyze PtdIns (1, 3, 4, 5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. Involved in endochondral ossification.
Subcellular Localization
Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Membrane; Translocates to membrane ruffles when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors such as the FC-gamma-RIIB receptor (FCGR2B). Tyrosine phosphorylation may also participate in membrane localization. Insulin specifically stimulates its redistribution from the cytosol to the plasma membrane. Recruited to the membrane following M-CSF stimulation. In activated spreading platelets, localizes with actin at filopodia, lamellipodia and the central actin ring.
Tissue Specificity
Widely expressed, most prominently in skeletal muscle, heart and brain. Present in platelets. Expressed in transformed myeloid cells and in primary macrophages, but not in peripheral blood monocytes.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug/vial
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 02.05.2024 

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