Anti-INPPL1 Antibody

Hersteller Boster
Kategorie
Typ Antibody Primary
Specific against other
Isotype IgG
Format Lyophilized
Applikationen WB, IHC, ELISA
Menge 100ug/vial
Host Rabbit
ArtNr BOS-A01790
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Clonality
Polyclonal
Description short
Rabbit IgG polyclonal antibody for INPPL1 detection. Tested with WB, IHC-P, Direct ELISA in Human, Mouse, Rat.
Specificity
No cross reactivity with other proteins.
Reactivity
Reacts with: human, mouse, rat
Application details
Western blot|0.1-0.5μ g/ml
Immunohistochemistry(Paraffin-embedded Section)|0.5-1μ g/ml
Direct ELISA|0.1-0.5μ g/ml
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
E. coli-derived human INPPL1 recombinant protein (Position: R1172-K1258).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification
Immunogen affinity purified.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage
At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Background
SH2-domain containing Phosphatidylinositol-3, 4, 5-trisphosphate 5-phosphatase 2 is an enzyme that in humans is encoded by the INPPL1 gene. The protein encoded by this gene is an SH2-containing 5'-inositol phosphatase that is involved in the regulation of insulin function. The encoded protein also plays a role in the regulation of epidermal growth factor receptor turnover and actin remodelling. Additionally, this gene supports metastatic growth in breast cancer and is a valuable biomarker for breast cancer.
Reference
1. Habib, T., Hejna, J. A., Moses, R. E., Decker, S. J. Growth factors and insulin stimulate tyrosine phosphorylation of the 51C/SHIP2 protein. J. Biol. Chem. 273: 18605-18609, 1998.
2. Sleeman, M. W., Wortley, K. E., Lai, K.-M. V., Gowen, L. C., Kintner, J., Kline, W. O., Garcia, K., Stitt, T. N., Yancopoulos, G. D., Wiegand, S. J., Glass, D. J. Absence of the lipid phosphatase SHIP2 confers resistance to dietary obesity. Nature Med. 11: 199-205, 2005.
3. Yu, J., Ryan, D. G., Getsios, S., Oliveira-Fernandes, M., Fatima, A., Lavker, R. M. MicroRNA-184 antagonizes microRNA-205 to maintain SHIP2 levels in epithelia. Proc. Nat. Acad. Sci. 105: 19300-19305, 2008.
Gene Name
INPPL1
Protein Name
Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 2
Gene Full Name
inositol polyphosphate phosphatase-like 1
Synonyms
Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 2; Inositol polyphosphate phosphatase-like protein 1; INPPL-1; Protein 51C; SH2 domain-containing inositol 5'-phosphatase 2; SH2 domain-containing inositol phosphatase 2; SHIP-2; INPPL1; SHIP2
Uniprot ID
O15357
Entrez GeneID
3636
Menge: 100ug/vial
Lieferbar: In stock
Listenpreis: 280,01 €
Preis: 280,01 €
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