Anti-COLLAGEN Type I COL1A1 Antibody Biotin Conjugated

Store vial at 4°C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Expiration date is one (1) year from date of opening.

Polyclonal

ELISA: 1:3, 000 - 1:6, 000
Flow Cytometry: User optimized
Fluorochrome Protein Value: 10-20
IHC: 1:50 - 1:200
IP: 1:100
WB: 1:3, 000 - 1:6, 000

Collagen alpha-1(I) chain

Collagen Type I from human and bovine placenta.

This product has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.  This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross-reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

1.0 mg/mL by UV absorbance at 280 nm

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Boster Bio Anti-COLLAGEN Type I COL1A1 Antibody Biotin Conjugated (Catalog # B00329). Tested in Dot blot, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.