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Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody Europäischer Partner

ArtNr BOS-B04429
Hersteller Boster
Menge 100 ug
Kategorie
Typ Antibody Monoclonal
Format Lyophilized
Applikationen WB, FC
Clon 11K8B1 IgG1
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1
Konjugat/Tag Biotin
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias IL-17E, IL-25, Interleukin 25, IL-25, Interleukin 17E, IL-17E
Lieferbar
Manufacturer - Category
Primary Antibodies, Monoclonal Antibodies
Storage Conditions
Store vial at 4°C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Observed Molecular Weight
20330 MW
Clonality
Monoclonal
Application notes
This purified antibody has been tested for use in Flow Cytometry and western blotting. Specific conditions for reactivity should be optimized by the end user.
Application Details
ELISA: 1:10, 000 - 1:50, 000
Flow Cytometry: 1µg/mL
Fluorochrome Protein Value: 10-20
WB: 0.5µg/mL
Manufacturer - Gene Name
IL25
Gene Full Name
Interleukin-25
Background
Anti-IL17E recognizes IL-17E (also known as IL25). IL-17E is produced and secreted by intra-epithelial lymphocytes, lung epithelial cells, alveolar macrophages, eosinophils, basophils, NKT cells, mast cells and cells of the gastro-intestinal tract and uterus. IL-17E stimulates the production of Th2-type cytokines. IL-17E limits Th17 cell development by inducing the expression of IL-13 by dendritic cells or by inhibiting IL-23 production by macrophages. Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. A number of cytokines, including IL-17E, have been implicated as oncogenes, reflecting the importance of these proteins in cell growth and survival. Anti-IL-17E cytokine antibody is ideal for investigators involved in Immunology, Signal Transduction research, Cancer and pro-inflammatory pathologies.
Immunogen
Anti-IL-17E (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17E produced in E.coli followed by hybridoma development.
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide
Purification
This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17E protein. A BLAST analysis was used to suggest cross-reactivity with IL-17E from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17E from other sources has not been determined.
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Manufacturer - Research Category
Cytokines, Immunology, Innate Immunity, Interleukins
Protein Function
Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
Subcellular Localization
Secreted.
Short Description
Boster Bio Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody (Catalog # B04429). Tested in Flow Cytometry, WB applications. This antibody reacts with Human.
Description
Boster Bio Anti-Human IL-17E Biotin Conjugated IL25 Monoclonal Antibody (Catalog # B04429). Tested in Flow Cytometry, WB applications. This antibody reacts with Human.
Tissue Specificity
Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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