Anti-Plasminogen PLG Antibody Peroxidase Conjugated

Primary Antibodies

90569 MW

Anti-Plasminogen has been tested by ELISA and western blot. This product is assayed against 1.0 µg of Plasminogen [Human Plasma] in a standard capture ELISA using ABTS (2, 2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1, 000 to 1:4, 000 of the reconstitution concentration is suggested for this product.

PLG

Plasmin is released as a zymogen called plasminogen (PLG) from the liver into the systemic circulation. Two major glycoforms of plasminogen are present in humans. Type II plasminogen is preferentially recruited to the cell surface over the type I glycoform. Conversely, type I plasminogen appears more readily recruited to blood clots. In circulation, plasminogen adopts a closed, activation resistant conformation. Upon binding to clots, or to the cell surface, plasminogen adopts an open form that can be converted into active plasmin by a variety of enzymes, including tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). Fibrin is a cofactor for plasminogen activation by tissue plasminogen activator. Urokinase plasminogen activator receptor (uPAR) is a cofactor for plasminogen activation by urokinase plasminogen activator. The conversion of plasminogen to plasmin involves the cleavage of the peptide bond between Arg-561 and Val-562. Deficiency in plasmin may lead to thrombosis, as clots are not degraded adequately. Plasminogen deficiency in mice leads to defective liver repair, defective wound healing, reproductive abnormalities. In humans, a rare disorder called plasminogen deficiency type I is caused by mutations of the PLG gene and is often manifested by ligneous conjunctivitis.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Cardiovascular, Cell Biology, Platelets, Protease Inhibitors, Proteolysis/Ubiquitin, Proteolytic Enzymes

Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.

Boster Bio Anti-Plasminogen PLG Antibody Peroxidase Conjugated (Catalog # H00702). Tested in ELISA, WB applications. This antibody reacts with Human.