Anti-p27Kip1 (Cyclin-dependent kinase inhibitor 1B) CDKN1B Rabbit Monoclonal Antibody, Clone#RM302

Primary Antibodies, Rabbit Monoclonal Antibodies

22073 MW

Immunohistochemistry (IHC): 1:100-1:200 dilution
WB: 1:500-1:1000 dilution.

Cyclin-dependent kinase inhibitor 1B

A peptide corresponding to the C-terminus of human p27Kip1.

Protein A affinity purified from an animal origin-free culture supernatant

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics and Nuclear Signaling

Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity).

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

This antibody reacts to human p27Kip1 (Cyclin-dependent kinase inhibitor 1B). It may also react to mouse p27Kip1, as predicted by immunogen homology.