Anti-KI67 MKI67 Rabbit Monoclonal Antibody, Clone#RM360

Rabbit IgG

358694 MW

Immunohistochemistry (IHC): 1:500 - 1:1000 dilution

WB: 1:1000-1:2000 dilution.

Proliferation marker protein Ki-67

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Boster Bio Anti-KI67 MKI67 Rabbit Monoclonal Antibody, Clone#RM360 (Catalog # M00254-5). Tested in IHC, WB applications. This antibody reacts with Human.