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Anti-Hsp27/HSPB1 Antibody Picoband™ (monoclonal, 3H3)

Anti-Hsp27/HSPB1 Antibody Picoband™ (monoclonal, 3H3)

Hersteller	Boster
Kategorie	Antibodies Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Immunocytochemistry Primary Antibodies
Typ	Antibody Monoclonal
Specific against	Human
Clon	3H3
Isotype	IgG1
Format	Lyophilized
Applikationen	WB, FC, IF, IHC, ICC
Menge	100 ug/vial
Host	Mouse
ArtNr	BOS-M00676-5
eClass 6.1	32160702
eClass 9.0	42030590
Lieferbar
Manufacturers Product Category	Primary Antibodies, Mouse Monoclonal Antibodies
Short Description	Boster Bio Anti-Hsp27/HSPB1 Antibody Picoband™ (monoclonal, 3H3) catalog # M00676-5. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Description	Boster Bio Anti-Hsp27/HSPB1 Antibody Picoband™ (monoclonal, 3H3) catalog # M00676-5. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Background	HSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. HSP27 gene is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Gene Name	HSPB1
Application Details	Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x10⁶ cells
Application Notes	Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Clonality	Monoclonal
Contents	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
Immunogen	E.coli-derived human Hsp27/HSPB1 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.
Cross-reactivity	No cross-reactivity with other proteins.
Storage	Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Gene Full Name	heat shock protein family B (small) member 1
Molecular Weight	27 kDa
Protein Function	Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.
Subcellular Localization	Nucleus. Spindle. Cytoplasm.
Tissue Specificity	Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 100 ug/vial

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Lieferung vsl. bis 30.05.2024

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