Anti-Desmin (DESM) Rabbit Monoclonal Antibody, Clone#RM234

Rabbit IgG

53536 MW

Immunohistochemistry (IHC): 1:1000-1:4000 dilution

WB: 1:1000-1:2000 dilution.

Desmin

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).

Boster Bio Anti-Desmin (DESM) Rabbit Monoclonal Antibody, Clone#RM234 (Catalog # M01948-2). Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Rat, Bovine