Anti-Involucrin (Squamous Cell Terminal Differentiation Marker) Monoclonal Antibody

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Monoclonal

IVL

This monoclonal antibody recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, This monoclonal antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Purified antibody with BSA and azide at 200ug/ml

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

Boster Bio Anti-Involucrin (Squamous Cell Terminal Differentiation Marker) Monoclonal Antibody (Catalog # M02628). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Gorilla, Owl monkey, Pig, Dog.

Keratinocytes of epidermis and other stratified squamous epithelia.