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Anti-Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Monoclonal Antibody Europäischer Partner

ArtNr BOS-M12092-1
Hersteller Boster
Menge 100 ug
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, FC, IF, IHC
Clon SPM116
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Monkey (Cynomolgus, Simian), Rabbit (Oryctolagus cuniculus), Chicken (Gallus gallus domesticus), Dog (Canine, Canis lupus familiaris), Cow (Bos taurus)
Host Mouse
Isotype IgG1 Kappa
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Keratin, type II cytoskeletal 2 oral;Cytokeratin-2P;CK-2P;K2P;Keratin-76;K76;Type-II keratin Kb9;KRT76;KRT2B, KRT2P;
Lieferbar
Manufacturer - Category
Primary Antibodies, Monoclonal Antibodies, 2279
Storage Conditions
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Observed Molecular Weight
65841 MW
Clonality
Monoclonal
Application Details
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml f)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Manufacturer - Gene Name
KRT76
Gene Full Name
Keratin, type II cytoskeletal 2 oral
Background
This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50' , 48, 46, 45, and 40kDa. This monoclonal antibody recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Immunogen
Human epidermal keratin
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purification
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G.
Concentration
Purified antibody with BSA and azide at 200ug/ml
Manufacturer - Research Category
Class I, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Signal Transduction, Subcellular Markers, Tags & Cell Markers
Protein Function
Probably contributes to terminal cornification.
Subcellular Localization
Cytoplasmic
Short Description
Boster Bio Anti-Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Monoclonal Antibody (Catalog # M12092-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken.
Description
Boster Bio Anti-Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Monoclonal Antibody (Catalog # M12092-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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