Anti-Phospho-Tryptophan Hydroxylase (Ser260) Antibody

Primary Antibodies

50985 MW

Specific for endogenous levels of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser260. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

TPH1

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser-260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Amino Acid Metabolism, Amino Acids, Cancer, Cancer Metabolism, Endocrine Metabolism, Energy Metabolism, Energy Transfer Pathways, Hormone Biosynthesis, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Neuroscience, Neurotransmitter, Pathways and Processes, Signal Transduction

Boster Bio Anti-Phospho-Tryptophan Hydroxylase (Ser260) Antibody (Catalog # P01626). Tested in WB applications. This antibody reacts with Human, Rat.

Isoform 2 seems to be less widely expressed than isoform 1.