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Anti-Phospho-ARHGAP22 S397 Antibody Europäischer Partner

ArtNr BOS-P10197
Hersteller Boster
Menge 50 ug
Kategorie
Typ Antibody
Format Liquid
Applikationen WB
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
Dry ice Yes
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias ARHGAP22 pS22, Rho-type GTPase-activating protein 22, RHOGAP2, Rho GTPase activating protein 22, rho GTPase-activating protein 22, ARHGAP 22, ARHGAP-22
Lieferbar
Manufacturer - Category
Primary Antibodies
Storage Conditions
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening.
Observed Molecular Weight
76779 MW
Manufacturer - Format
Liquid (sterile filtered)
Clonality
Polyclonal
Application notes
Anti-ARHGAP22 pS22 antibody is tested in Western Blot and ELISA useful for Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77.8 kDa corresponding to the appropriate cell lysate or extract.
Application Details
ELISA: 1:20, 000 - 1:60, 000
IHC: 1:100-1:500
IF Microscopy: 1:100-1:500
WB: 1 µg/ml
Manufacturer - Gene Name
ARHGAP22
Gene Full Name
Rho GTPase-activating protein 22
Background
ARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. Anti-ARHGAP22 [p Ser22] antibody is ideal for researchers interested in Diabetes Research, Lipid and Metabolism research.
Immunogen
ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS22 region of ARHGAP22.
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol
Purification
Anti-ARHGAP22 pS22 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for phosphorylated ARHGAP22 at Serine 22. It also recognizes the S397->A mutation but not the S22->A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100% sequence homology. Cross-reactivity with ARHGAP22 pS22 from other sources has not been determined.
Concentration
1.07 mg/ml by UV absorbance at 280 nm
Manufacturer - Research Category
G Protein Signaling, Regulators, Signal Transduction, Signaling Pathway, Small G Proteins
Protein Function
Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter (By similarity).
Subcellular Localization
Cytoplasm. Nucleus. Mainly cytoplasmic. Some fraction is nuclear (By similarity).
Short Description
Boster Bio Anti-Phospho-ARHGAP22 S397 Antibody (Catalog # P10197). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Description
Boster Bio Anti-Phospho-ARHGAP22 S397 Antibody (Catalog # P10197). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 ug
Lieferbar: In stock
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