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Human Neurotrophin-3 PicoKine ELISA Kit

Hersteller Boster
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96wells/kit, with removable strips.
ArtNr BOS-EK0472
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Gene name
NTF3
Assay range
15.6pg/ml-1000pg/ml
Components needed
  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
    • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μ l of purified acetic acid or 700μ l of concentrated hydrochloric acid to 1000ml H2
    • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Conjugate
No
Description
For quantitative detection of human Neurotrophin-3 in cell culture supernates and serum.
Uniprot
P20783
Immunogen
Expression system for standard: sf21; Immunogen sequence: Y139-T257
Storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reactivity
There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
Sensitivity
<2pg/ml
Kit components
10ng/tube
Sample type
cell culture supernates and serum.
Predicted reactivity
Bovine, Chicken, Horse, Monkey, Rabbit
Assay procedure step1
1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml
Background
Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1
Research category
|neuroscience|neurology process|growth and development|neurotrophins
Synonyms
Neurotrophin-3; NT-3; HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factor; NTF3;
Gene full name
Neurotrophin-3
MW
29355 MW
Protein function
Seems to promote the survival of visceral and proprioceptive sensory neurons.
Subcellular localization
Secreted.
Tissue specificity
Brain and peripheral tissues.
Protein name
Neurotrophin-3
Intra inter assay cv
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|93|357|657|146|375|732
Standard deviation|4|17.5|34.2|7.88|22.9|47.58
CV(%)|4.3|4.9|5.2|5.4|6.1|6.5
Sample data
Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000
O.D.|0.014|0.073|0.148|0.277|0.561|0.870|1.686|2.529
Tmb Incubation time
20-25min
Principle
monoclonal antibody from mouse|polyclonal antibody from goat
Normal range citations
21085492

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Menge: 96wells/kit, with removable strips.
Lieferbar: In stock
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