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Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®

Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®

Hersteller	Boster
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis By Cell Type Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Heart & Blood Cancer
Typ	Elisa-Kit
Specific against	Human
Applikationen	ELISA
Menge	96 wells/kit, with removable strips.
ArtNr	BOS-EK0525
Targets	TNF
eClass 6.1	32160605
eClass 9.0	32160605
Lieferbar
Manufacturers Product Category	PicoKine® ELISA Kits
Manufacturers Research Category	Atherosclerosis, Cancer, Cardiovascular, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Metabolism, Signal Transduction, TNF Superfamily, Vascular Inflammation
Short Description	Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine® (96 Tests). Quantitate Human TNF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.
Description	Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine® (96 Tests). Quantitate Human TNF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.
Background	Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
Gene Name	TNF
Application Details	Dilution ratio of 1:1, concentration in serum and plasma is around 100 pg/ml.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: V77-L233
Cross-reactivity	There is no detectable cross-reactivity with other relevant proteins.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Capture antibody\|Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Anticoagulant	heparin, EDTA or citrate
Gene Full Name	Tumor necrosis factor
Molecular Weight	25644 MW
Protein Function	Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918)..
Subcellular Localization	Cell membrane ; Single-pass type II membrane protein.
Protein Name	Tumor necrosis factor
Sequence Similarities	Belongs to the tumor necrosis factor family.

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