Human TNF alpha PicoKine ELISA Kit

Hersteller Boster
Kategorie Produkte / ELISA
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96 Wells
ArtNr BOS-EK0525
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Gene name
TNF
Assay range
15.6pg/ml-1000pg/ml(cellculturesupernates), 7.8pg/ml-500pg/ml(serum, plasma)
Components needed
  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
    • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μ l of purified acetic acid or 700μ l of concentrated hydrochloric acid to 1000ml H2
    • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Conjugate
No
Description
For quantitative detection of human TNF alpha in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Uniprot
P01375
Immunogen
Expression system for standard: E.coli, V77-L233
Storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reactivity
There is no detectable cross-reactivity with other relevant proteins.
Sensitivity
<1pg/ml
Kit components
10ng/tube
Sample type
cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Anticoagulant
heparin, EDTA or citrate
Assay procedure step1
1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml
Background
Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
Research category
|immunology|innate immunity|cytokines|tnf superfamily| signal transduction|growth factors/hormones|tnf| cancer|growth factors| cardiovascular|atherosclerosis|vascular inflammation|inflammatory mediators| metabolism|types of disease|metabolic disorders
Synonyms
Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; TNF; TNFA, TNFSF2;
Gene full name
Tumor necrosis factor
MW
25644 MW
Protein function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918). .
Subcellular localization
Cell membrane ; Single-pass type II membrane protein .
Protein name
Tumor necrosis factor
Sequence similarities
Belongs to the tumor necrosis factor family.
Intra inter assay cv
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|93|327|608|102|319|613
Standard deviation|5.1|15.4|31|7.65|15.3|35
CV(%)|5.5|4.7|5.1|7.5|4.8|5.7
Sample data
Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000
O.D.|0.065|0.118|0.167|0.272|0.510|0.839|1.582|2.412
Tmb Incubation time
20-25min
Principle
monoclonal antibody from mouse|polyclonal antibody from goat
Normal range citations
24812899
Menge: 96 Wells
Lieferbar: In stock
Listenpreis: 227,27 €
Rabatt: -10%
Preis: 204,54 €
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Sicherheitsdatenblatt

BOS-EK0525.pdf

Arbeitsanleitung

BOS-EK0525.pdf