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Human CD40/TNFRSF5 ELISA Kit PicoKine®

Human CD40/TNFRSF5 ELISA Kit PicoKine®

ArtNr

BOS-EK0702

Hersteller

Boster

Menge

96 wells/kit, with removable strips.

Kategorie

Typ

Elisa-Kit

Applikationen

ELISA

Specific against

Human (Homo sapiens)

Sensitivity

<1 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CDw40;CD40;CD40;TNFRSF5;

Lieferbar

Manufacturer - Category

PicoKine® ELISA Kits

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular weight

30619 MW

Manufacturer - Gene Name

CD40

Gene Full Name

Tumor necrosis factor receptor superfamily member 5

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Sample Data

Concentration (pg/ml)

Manufacturer - Specificity

Natural and recombinant human CD40

Intra Assay Consistency & Inter Assay Consistency

Intra-Assay Precision

Inter-Assay Precision

Sample

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Background	CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
Immunogen	Expression system for standard: NS0; Immunogen sequence: E21-R193
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Research Category	Adaptive Immunity, Atherosclerosis, B Cells, Cardiovascular, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells, Vascular Inflammation
Protein Name	Tumor necrosis factor receptor superfamily member 5
Protein Function	Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.
Subcellular Localization	Isoform I: Cell membrane; Single-pass type I membrane protein.
Description	Human CD40/TNFRSF5 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD40 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	B-cells and in primary carcinomas.
Sequence Similarities	Contains 4 TNFR-Cys repeats.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK0702.pdf

BOS-EK0702-datasheet.pdf

BOS-EK0702-safety-datasheet.pdf