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Human Galectin-1 ELISA Kit PicoKine®

Human Galectin-1 ELISA Kit PicoKine®

ArtNr	BOS-EK0762
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Oncology ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Immunostaining Lymphatic System Cancer
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<15 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Galectin-1,Gal-1,14 kDa laminin-binding protein,HLBP14,14 kDa lectin,Beta-galactoside-binding lectin L-14-I,Galaptin,HBL,HPL,Lactose-binding lectin 1,Lectin galactoside-binding soluble 1,Putative MAPK-activating protein PM12,S-Lac lectin 1,LGALS1
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	14716 MW
Gene Name	LGALS1
Gene Full Name	Galectin-1
Manufacturer - Specificity	Natural and recombinant human Galectin-1
Background	Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Its expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. Additionally, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: A2-D135
Manufacturer - Research Category	Cell Biology
Protein Name	Galectin-1
Protein Function	May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
Subcellular Localization	Secreted, extracellular space, extracellular matrix.
Description	Human Galectin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sample Data	Concentration (pg/ml) \| 0 \| 156 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 O.D. \| 0.047 \| 0.119 \| 0.144 \| 0.252 \| 0.516 \| 0.892 \| 1.609 \| 2.092
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 232 \| 1786 \| 5647 \| 229 \| 1822 \| 5807 Standard deviation \| 13.45 \| 121.44 \| 355.76 \| 13.96 \| 147.58 \| 476.17 CV (%) \| 5.8% \| 6.8% \| 8.1% \| 6.1% \| 8.1% \| 8.2%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 232 \| 226 \| 230 \| 221 \| 227 \| 4.2 \| 1.8% Sample 2 \| 1786 \| 1592 \| 1746 \| 1838 \| 1740 \| 91.73 \| 5.2% Sample 3 \| 5647 \| 5665 \| 6073 \| 5299 \| 5671 \| 274.13 \| 4.8% *number of samples for each test n=16.
Capture antibody and Detection antibody	A polyclonal antibody from goat\|a biotinylated detection polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Alias	Galectin-1, Gal-1, 14 kDa laminin-binding protein, HLBP14, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, LGALS1
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK0762.pdf

BOS-EK0762-datasheet.pdf

BOS-EK0762-safety-datasheet.pdf