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Human IL-31/Interleukin-31 ELISA Kit PicoKine®

Human IL-31/Interleukin-31 ELISA Kit PicoKine®

ArtNr	BOS-EK0979
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Oncology Neuroscience Neural Lineage Markers Neural Transcription Factors Targets for Rodent Neurosciences Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Skin Cancer Hepatic Cancer Immunostaining Bone Cancer
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Interleukin-31,IL-31,IL31
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	18205 MW
Gene Name	IL31
Gene Full Name	Interleukin-31
Manufacturer - Specificity	Natural and recombinant human IL-31
Background	Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. This gene is mapped to 12q24.31. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma. IL31 may function in skin immunity.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: S24-T164
Manufacturer - Research Category	Cytokines, Immunology, Innate Immunity, Interleukins
Protein Name	Interleukin-31
Protein Function	Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.
Subcellular Localization	Secreted.
Description	Human IL-31 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL31 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sample Data	Concentration (pg/ml) \| 0 \| 62.5 \| 125 \| 250 \| 500 \| 1000 \| 2000 \| 4000 O.D. \| 0.004 \| 0.142 \| 0.197 \| 0.316 \| 0.586 \| 0.984 \| 1.446 \| 2.096
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 134 \| 804 \| 1907 \| 140 \| 740 \| 1811 Standard deviation \| 8.04 \| 44.22 \| 122.04 \| 10.5 \| 52.54 \| 130.39 CV (%) \| 6% \| 5.5% \| 6.4% \| 7.5% \| 7.1% \| 7.2%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 134 \| 122 \| 129 \| 116 \| 125 \| 6.83 \| 5.4% Sample 2 \| 804 \| 765 \| 827 \| 741 \| 784 \| 33.38 \| 4.2% Sample 3 \| 1907 \| 1624 \| 1623 \| 1803 \| 1739 \| 121.45 \| 6.9% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK0979.pdf

BOS-EK0979-datasheet.pdf

BOS-EK0979-safety-datasheet.pdf