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Human TGF-Beta 3 ELISA Kit PicoKine®

Human TGF-Beta 3 ELISA Kit PicoKine®

ArtNr

BOS-EK1103

Hersteller

Boster

Menge

96 wells/kit, with removable strips.

Kategorie

Typ

Elisa-Kit

Applikationen

ELISA

Specific against

Human (Homo sapiens)

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Transforming growth factor beta-3;TGF-beta-3;Latency-associated peptide;LAP;TGFB3;

Lieferbar

Manufacturer - Category

PicoKine® ELISA Kits

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular weight

47328 MW

Manufacturer - Gene Name

TGFB3

Gene Full Name

Transforming growth factor beta-3

Sample Type

cell culture supernatants, serum, plasma (EDTA) and urine.

Sample Data

Concentration (pg/ml)

Manufacturer - Specificity

Natural and recombinant human TGF-beta 3

Intra Assay Consistency & Inter Assay Consistency

Intra-Assay Precision

Inter-Assay Precision

Sample

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	EDTA
Activating Reagent	TGF beta 3 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant TGF beta 3. Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H₂O. Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H₂O, add H₂O to adjust volume to 100ml.
Sample Activation Procedure	Cell culture supernatants, urine: add activating reagent pro rata, i.e. add 20?l of Solution A into 100?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6. Serum, plasma(EDTA): add activating reagent pro rata, i.e. add 20?l of Solution A into 40?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant TGF-beta 3. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.
Background	Transforming growth factor beta 3 (TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin (CTNNB1) via nuclear phospho-Smad2 and Smad4.
Immunogen	Expression system for standard: NS0; Immunogen sequence: A301-S412
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Research Category	Angiogenesis, Cancer, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta
Protein Name	Transforming growth factor beta-3
Protein Function	Involved in embryogenesis and cell differentiation.
Subcellular Localization	Secreted.
Description	Human TGF-Beta 3 ELISA Kit PicoKine® (96 Tests). Quantitate Human TGFB3 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sequence Similarities	Belongs to the TGF-beta family.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK1103.pdf

BOS-EK1103-datasheet.pdf

BOS-EK1103-safety-datasheet.pdf