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Human CEACAM1/Cd66A ELISA Kit PicoKine®

Human CEACAM1/Cd66A ELISA Kit PicoKine®

ArtNr	BOS-EK1361
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Oncology Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism By Cell Type Carcinoma Hematology Leukemia Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Testicular & Prostate Cancer Gallbladder Cancer Gastric Cancer Pancreatic Cancer Thyroid Cancer Hepatic Cancer Immunostaining Other Lipid\Cholesterol Metabolism Modulators
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
Sequence	Belongs to the immunoglobulin superfamily. CEA family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Carcinoembryonic antigen-related cell adhesion molecule 1,Biliary glycoprotein 1,BGP-1,CD66a,CEACAM1,BGP,BGP1
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	57560 MW
Gene Name	CEACAM1
Gene Full Name	Carcinoembryonic antigen-related cell adhesion molecule 1
Manufacturer - Specificity	Natural and recombinant human CEACAM1
Background	CEACAM1, also known as CD66a (Cluster of Differentiation 66a), is a human glycoprotein encoded by a gene. This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 is mapped to 19q13.2 in human. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.
Immunogen	Expression system for standard: NS0; Immunogen sequence: Q35-G428
Manufacturer - Research Category	Cancer, Cell Type Markers, Human Protein, Microbiology, Oncoproteins/Suppressors, Protein, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor Suppressors, Tumor-Associated Antigens
Protein Name	Carcinoembryonic antigen-related cell adhesion molecule 1
Subcellular Localization	Isoform 1: Cell membrane; Single-pass type I membrane protein.
Description	Human CEACAM1/Cd66A ELISA Kit PicoKine® (96 Tests). Quantitate Human CEACAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sequence Similarities	Belongs to the immunoglobulin superfamily. CEA family.
Sample Data	Concentration (pg/ml) \| 0 \| 312 \| 625 \| 10, 000000 \| 10, 00000 \| 10, 0000 \| 10, 000 \| 20, 000 O.D. \| 0.096 \| 0.220 \| 0.286 \| 0.505 \| 0.814 \| 1.279 \| 1.970 \| 2.589
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 463 \| 2835 \| 9439 \| 432 \| 3010 \| 9810 Standard deviation \| 32.41 \| 150.25 \| 453.07 \| 35.42 \| 207.69 \| 529.74 CV (%) \| 7% \| 5.3% \| 4.8% \| 8.2% \| 6.9% \| 5.4%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 463 \| 407 \| 434 \| 425 \| 432 \| 20.24 \| 4.6% Sample 2 \| 2835 \| 2793 \| 2564 \| 2668 \| 2715 \| 106.64 \| 3.9% Sample 3 \| 9439 \| 8061 \| 8668 \| 8372 \| 8635 \| 511.4 \| 5.9% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	20 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK1361.pdf

BOS-EK1361-datasheet.pdf

BOS-EK1361-safety-datasheet.pdf