Human CD5L/CT-2 ELISA Kit PicoKine®

Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4

heparin or EDTA

Cell culture supernatants: add activating reagent pro rate, i.e. add 20?l of Solution A into 40?l of sample, 10 min later, add 20?l of Solution B. PH7.0-7.6.
Serum: add activating reagent pro rate, i.e. add 20?l of Solution A into 40?l of sample, 10 min later, add 20?l of Solution B. PH7.0-7.6. Prior to the assay, the activated sample requires a 2000-fold dilution. Add 10?l of activated sample into 390?l wash buffer (1 : 40). And then add 4?l of above diluted reagent into 196?l sample diluent buffer ( 1 : 50).
Plasma(heparin, EDTA): add activating reagent pro rate, i.e. add 20?l of Solution A into 40?l of sample, 10 min later, add 20?l of Solution B. PH7.0-7.6. Prior to the assay, the activated sample requires a 4000-fold dilution. Add 10?l of activated sample into 790?l wash buffer (1 : 80). And then add 4?l of above diluted reagent into 196?l sample diluent buffer (1 : 50).Optimal dilutions should be determined by end users.
It is unnecessary to activate the recombinant CD5L.
Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Expression system for standard: NS0; Immunogen sequence: S20-G347

Apoptosis, Cell Biology, Intracellular

Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflammed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates to obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity- related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). Acts as a key regulator of metabolic switch in T-helper Th17 cells. Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation. CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes. Participates in obesity- associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies (By similarity). Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis (PubMed:24295828). Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy (PubMed:16030018, PubMed:24223991, PubMed:24583716, PubMed:25713983).

Human CD5L / CT 2 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD5L in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Contains 3 SRCR domains.