Human Caspase 3/CASP3 ELISA Kit PicoKine®

Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4

Caspase-3 is a caspase protein that is encoded by the CASP3 gene. This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Caspase-3

Cytoplasm.

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.