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Human Afamin ELISA Kit PicoKine®

Human Afamin ELISA Kit PicoKine®

ArtNr

BOS-EK1487

Hersteller

Boster

Menge

96 wells/kit, with removable strips.

Kategorie

Typ

Elisa-Kit

Applikationen

ELISA

Specific against

Human (Homo sapiens)

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Afamin;Alpha-albumin;Alpha-Alb;AFM;ALB2, ALBA;

Lieferbar

Manufacturer - Category

PicoKine® ELISA Kits

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular weight

69069 MW

Manufacturer - Gene Name

AFM

Gene Full Name

Afamin

Sample Type

cell culture supernatants, serum and urine.

Sample Data

Concentration (pg/ml)

Manufacturer - Specificity

Natural and recombinant human Afamin

Intra Assay Consistency & Inter Assay Consistency

Intra-Assay Precision

Inter-Assay Precision

Sample

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Background	Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.
Immunogen	Expression system for standard: NS0; Immunogen sequence: L22-N599
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Research Category	Cardiovascular, Cofactors, Vitamins/Minerals, Metabolism, Pathways and Processes, Serum Proteins, Signal Transduction, Vitamins/Minerals
Protein Name	Afamin
Protein Function	Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier.
Subcellular Localization	Secreted.
Description	Human Afamin ELISA Kit PicoKine® (96 Tests). Quantitate Human AFM in cell culture supernatants, serum and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK1487.pdf

BOS-EK1487-datasheet.pdf

BOS-EK1487-safety-datasheet.pdf