Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine®

PicoKine® ELISA Kits

68418 MW

Cholinesterase

Butyrylcholinesterase is a nonspecific cholinesterase enzyme that in humans is encoded by the BCHE gene. This gene is mapped to 3q26. It is a member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine.

Alzheimer's Disease, Drug Metabolism, Metabolic Signaling Pathways, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Neurotransmitter, Pathways and Processes, Signal Transduction

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.

Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine® (96 Tests). Quantitate Human BCHE in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Intra-Assay Precision | Inter-Assay Precision
Sample | 1 | 2 | 3 | 1 | 2 | 3
n | 16 | 16 | 16 | 24 | 24 | 24
Mean (pg/ml) | 1205 | 4078 | 24826 | 1297 | 4376 | 24447
Standard deviation | 66.27 | 322.16 | 1613.69 | 80.41 | 380.71 | 1662.39
CV (%) | 5.5% | 7.9% | 6.5% | 6.2% | 8.7% | 6.8%

monoclonal antibody from mouse|polyclonal antibody from goat

40 ng/tube

Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.