Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Tryptase/TPSAB1, B2

Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

Cell Biology, Immunology, Innate Immunity, Interspecies Interaction, Microbiology, Proteolysis/Ubiquitin, Proteolytic Enzymes, Secreted Molecules

Secreted. Released from the secretory granules upon mast cell activation.

TPSAB1: Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 ispredominant. Isoform 2 is expressed in aorta, spleen, and breasttumor, with highest levels in the endothelial cells of some bloodvessels surrounding the aorta, as well as those surrounding thetumor and low levels, if any, in mast cells (at protein level).

Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000
O.D. | 0.068 | 0.113 | 0.212 | 0.301 | 0.459 | 0.714 | 1.236 | 1.861

Catalog number | Description | Quantity | Storage of opened/reconstituted material
EZ0898-CA | Rabbit anti- human Tryptase/TPSAB1, B2 polyclonal antibody (Capture Antibody) | 500 µ l, 0.4 mg/mL
(recommended dilution 1:100)
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0898-DA | Biotinylated goat anti- human Tryptase/TPSAB1, B2 polyclonal antibody (Detection Antibody) | 500 µ l, 12 µ g/mL
(recommended dilution 1:100)
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µ l (recommended dilution 1:100)
EK0898-ST | Lyophilized recombinant human Tryptase/TPSAB1, B2 standard | 10 ng/tubex3
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit.

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)