Human CEA ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

carcinoembryonic antigen-related cell adhesion molecule 5

Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180, 000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

Cancer, Cell Type Markers, Human Protein, Microbiology, Oncoproteins/Suppressors, Protein, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor Suppressors, Tumor-Associated Antigens

Cell membrane.

Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000
O.D. | 0.011 | 0.056 | 0.111 | 0.210 | 0.381 | 0.713 | 1.296 | 1.967

Catalog number | Description | Quantity | Storage of opened/reconstituted material
EZ0904-CA | Rabbit anti- human CEACAM5 polyclonal antibody (Capture Antibody) | 500 µ l, 0.4 mg/mL
(recommended dilution 1:100)
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0904-DA | Biotinylated goat anti- human CEACAM5 polyclonal antibody (Detection Antibody) | 500 µ l, 4 µ g/mL
(recommended dilution 1:100)
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µ l (recommended dilution 1:100)
EK0904-ST | Lyophilized recombinant human CEACAM5 standard | 20 ng/tubex3
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit.

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)