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Human SP-D/Surfactant protein D EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Human SP-D/Surfactant protein D EZ-Set™ ELISA Kit (DIY Antibody Pairs)

ArtNr	BOS-EZ1171
Hersteller	Boster
Menge	5 plates/kit
Kategorie	Oncology ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<20 pg/ml
Sequence	Belongs to the SFTPD family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Pulmonary surfactant-associated protein D,PSP-D,SP-D,Collectin-7,Lung surfactant protein D,SFTPD,COLEC7,PSPD,SFTP4
Lieferbar
Manufacturer - Category	EZ-Set™ ELISA Kits (Ab Pairs)
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Molecular Weight	37728 MW
Calculated Molecular weight	37728 MW
Gene Name	SFTPD
Gene Full Name	Pulmonary surfactant-associated protein D
Manufacturer - Specificity	Natural and recombinant human SP-D
Background	Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What’s more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.
Immunogen	Expression system for standard: NS0; Immunogen sequence: A21-F375
Manufacturer - Research Category	Calcium Binding Proteins, Calcium Signaling, Cancer, Cancer Metabolism, Cell Biology, Defensin, Human Protein, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolism, Microbiology, Oxidative Stress, Pathways and Processes, Protein, Redox Metabolism, Signal Transduction, Signaling Pathway
Protein Name	Pulmonary surfactant-associated protein D
Protein Function	Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Subcellular Localization	Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
Description	Human SP-D/Surfactant protein D ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human SFTPD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Tissue Specificity	Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes).
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sequence Similarities	Belongs to the SFTPD family.
Sample Data	Concentration (pg/ml) \| 0 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 \| 20000 \| 40000 O.D. \| 0.049 \| 0.188 \| 0.306 \| 0.514 \| 0.979 \| 1.383 \| 1.708 \| 2.056
Kit Components	Catalog number \| Description \| Quantity \| Storage of opened/reconstituted material EZ1171-CA \| Goat anti-human SP-D/Surfactant protein D monoclonal antibody (Capture Antibody) \| 500 µ l, 4 µ g/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1171-DA \| Biotinylated goat anti-human SP-D/Surfactant protein D polyclonal antibody (Detection Antibody) \| 500 µ l (recommended dilution 1:100) AR1103 \| Avidin-Biotin-Peroxidase Complex (ABC) \| 500 µ l (recommended dilution 1:100) EZ1171-ST \| Lyophilized recombinant human SP-D/Surfactant protein D standard \| 40 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.
Anticoagulant	heparin or EDTA
Material Required But Not Provided	1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T). Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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BOS-EZ1171-datasheet.pdf

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