Monkey primate TGF Alpha ELISA Kit PicoKine®

polyclonal antibody from goat|polyclonal antibody from goat

10 ng/tube

Transforming growth factor alpha (TGF-alpha) is upregulated in some monkey primate cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene (TGFA) maps to monkey primate chromosome 2 close to the breakpoint of the t (2; 8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic monkey primate TGF-alpha showed similar immunoreactivity when compared with monkey primate TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Protransforming growth factor alpha

Transforming growth factor alpha: Secreted, extracellular space.

Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.