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Mouse Leptin Receptor ELISA Kit PicoKine®

Mouse Leptin Receptor ELISA Kit PicoKine®

ArtNr	BOS-EK0440
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Oncology Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Neural Transcription Factors Neural Signaling Neural Adhesion Molecules By Cell Type Carcinoma Hematology Leukemia Obesity ELISA Endocrine Regulation of Lipid Metabolism ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Immunostaining Neuronal Cell Markers Progenitor Markers
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	<5 pg/ml
Sequence	Belongs to the type I cytokine receptor family. Type 2 subfamily.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Leptin receptor,LEP-R,B219,OB receptor,OB-R,CD295,Lepr,Db,Obr
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	130789 MW
Gene Name	LEPR
Gene Full Name	Leptin receptor
Manufacturer - Specificity	Natural and recombinant mouse Leptin receptor
Background	Leptin receptor (or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene (LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.
Immunogen	Expression system for standard: NS0; Immunogen sequence: L22-G839
Manufacturer - Research Category	Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction, Structures
Protein Name	Leptin receptor
Protein Function	Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
Subcellular Localization	Cell membrane; Single-pass type I membrane protein.
Description	Mouse Leptin Receptor ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lepr in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Isoform A: highest level of expression in lung and kidney, also present in kidney, heart, brain, spleen, liver, muscle, choroid plexus and hypothalamus. Isoform B: highest level of expression in hypothalamus and lower level in brain, testes and adipose tissue. Isoform E: expressed in adipose tissue, hypothalamus, heart, and testes.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sequence Similarities	Belongs to the type I cytokine receptor family. Type 2 subfamily.
Sample Data	Concentration (pg/ml) \| 0 \| 156 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 O.D. \| 0.016 \| 0.070 \| 0.128 \| 0.248 \| 0.458 \| 0.817 \| 1.452 \| 2.011
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 222 \| 1398 \| 4930 \| 200 \| 1476 \| 5345 Standard deviation \| 15.09 \| 86.87 \| 359.89 \| 15.8 \| 110.7 \| 427.6 CV (%) \| 6.8% \| 6.1% \| 7.3% \| 7.9% \| 7.5% \| 8%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 222 \| 189 \| 209 \| 209 \| 207 \| 11.79 \| 5.6% Sample 2 \| 1398 \| 1396 \| 1505 \| 1381 \| 1420 \| 49.51 \| 3.4% Sample 3 \| 4930 \| 5654 \| 5332 \| 5633 \| 5387 \| 293.12 \| 5.4% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from rat\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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BOS-EK0440.pdf

BOS-EK0440-datasheet.pdf

BOS-EK0440-safety-datasheet.pdf