Mouse Thrombomodulin ELISA Kit PicoKine®

PicoKine® ELISA Kits

Mouse Thrombomodulin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Thbd in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.

Thrombomodulin (TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor (TAFI, aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene (THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome (aHUS).

For serum, dilution ratio is 1:20 and concentration is 12296-35284 pg/ml.
For plasma, dilution ratio is 1:20 and concentration is 9384-29772 pg/ml.

There is no detectable cross-reactivity with other relevant proteins.

monoclonal antibody from rat|polyclonal antibody from goat

heparin, EDTA or citrate

61868 MW

Membrane; Single-pass type I membrane protein.

Thrombomodulin