Mouse Thrombomodulin PicoKine ELISA Kit

Hersteller Boster
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 96 Wells
ArtNr BOS-EK1119
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Gene name
Thbd
Assay range
62.5pg/ml-4000pg/ml
Components needed
  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
    • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μ l of purified acetic acid or 700μ l of concentrated hydrochloric acid to 1000ml H2
    • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Conjugate
No
Description
For quantitative detection of mouse Thrombomodulin in cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.
Uniprot
P15306
Immunogen
Expression system for standard: NSO; Immunogen sequence: L17-S517
Storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reactivity
There is no detectable cross-reactivity with other relevant proteins.
Sensitivity
<10pg/ml
Kit components
10ng/tube
Sample type
cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.
Anticoagulant
heparin, EDTA or citrate
Assay procedure step1
4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml
Background
Thrombomodulin(TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor(TAFI, aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene(THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome(aHUS).
Research category
|cardiovascular|blood|coagulation|regulatory| stem cells|endothelial progenitors|endothelial markers| kits/ lysates/ other|elisa kits|adhesion molecules elisa kits| cardiovascular|cardiovascular markers|cell markers|endothelial cells
Synonyms
Thrombomodulin; TM; Fetomodulin; CD141; Thbd;
Gene full name
Thrombomodulin
MW
61868 MW
Protein function
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
Subcellular localization
Membrane; Single-pass type I membrane protein.
Tissue specificity
Endothelial cells are unique in synthesizing thrombomodulin.
Protein name
Thrombomodulin
Sequence similarities
Contains 1 C-type lectin domain.
Intra inter assay cv
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|257|1942|3568|366|2158|3643
Standard deviation|17.5|101.1|167.7|31.8|153.2|251.4
CV(%)|6.8|5.2|4.7|8.7|7.1|6.9
Sample data
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000
O.D.|0.007|0.155|0.284|0.476|0.806|1.313|1.818|2.166
Tmb Incubation time
20-25min
Principle
monoclonal antibody from rat|polyclonal antibody from goat

Sicherheitsdatenblatt

BOS-EK1119.pdf

Arbeitsanleitung

BOS-EK1119.pdf

Menge: 96 Wells
Lieferbar: In stock
Listenpreis: 369,00 €
Preis: 369,00 €
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