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Mouse CD70/TNFSF7/cd27L ELISA Kit PicoKine®

Mouse CD70/TNFSF7/cd27L ELISA Kit PicoKine®

ArtNr

BOS-EK1310

Hersteller

Boster

Menge

96 wells/kit, with removable strips.

Kategorie

Typ

Elisa-Kit

Applikationen

ELISA

Specific against

Mouse (Murine, Mus musculus)

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

CD70 antigen;CD27 ligand;CD27-L;Tumor necrosis factor ligand superfamily member 7;CD70;Cd70;Cd27l, Cd27lg, Tnfsf7;

Lieferbar

Manufacturer - Category

PicoKine® ELISA Kits

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular weight

21919 MW

Manufacturer - Gene Name

CD70

Gene Full Name

CD70 antigen

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Sample Data

Concentration (pg/ml)

Manufacturer - Specificity

Natural and recombinant mouse CD70

Intra Assay Consistency & Inter Assay Consistency

Intra-Assay Precision

Inter-Assay Precision

Sample

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1
Capture antibody and Detection antibody	monoclonal antibody from rat\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Background	CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.
Immunogen	Expression system for standard: NS0; Immunogen sequence: S45-P195
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Research Category	Cancer, Cytokines, Growth Factors, Hematopoietic Progenitors, Immunology, Innate Immunity, Lymphoid, Stem Cells, T Lymphocytic Lineage, TNF Superfamily
Protein Name	CD70 antigen
Protein Function	Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Subcellular Localization	Membrane; Single-pass type II membrane protein.
Description	Mouse CD70/TNFSF7/cd27L ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd70 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Very low level of expression. Detected in splenocytes and thymocytes.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK1310.pdf

BOS-EK1310-datasheet.pdf

BOS-EK1310-safety-datasheet.pdf