Mouse AFP ELISA Kit PicoKine®

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

alpha fetoprotein

alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. This gene is mapped to 5 E1; 5 44.77 cM. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.

Cancer, Cardiovascular, Developmental Biology, Organogenesis, Serum Proteins, Tumor Antigens, Tumor Biomarkers

Binds estrogens, fatty acids and metals.

Mouse AFP ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Afp in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

cell culture supernatants, serum and plasma (heparin, EDTA).

Intra-Assay Precision | Inter-Assay Precision
Sample | 1 | 2 | 3 | 1 | 2 | 3
n | 16 | 16 | 16 | 24 | 24 | 24
Mean (pg/ml) | 787 | 2378 | 9095 | 804 | 2193 | 8576
Standard deviation | 44.07 | 149.81 | 436.56 | 49.84 | 144.73 | 471.68
CV (%) | 5.6% | 6.3% | 4.8% | 6.2% | 6.6% | 5.5%

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

20 ng/tube

Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.