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Human CTRC/Chymotrypsin-C ELISA Kit PicoKine®

Human CTRC/Chymotrypsin-C ELISA Kit PicoKine®

ArtNr	BOS-EK2001
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Oncology By Cell Type ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Chymotrypsin-C,Caldecrin,CTRC,CLCR
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	CTRC
Gene Full Name	chymotrypsin C
Manufacturer - Specificity	Natural and recombinant human CTRC
Background	Chymotrypsin C (CTRC), also known as caldecrin, is a serine protease and a member of the peptidase S1 family. This gene is mapped to 1p36.21. This gene encodes a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.
Immunogen	Expression system for standard: CHO; Immunogen sequence: V30-L268
Protein Name	Chymotrypsin-C
Protein Function	Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity.
Subcellular Localization	Extracellular region.
Description	Human CTRC ELISA Kit PicoKine® (96 Tests). Quantitate Human CTRC in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Pancreas.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Sample Data	Concentration (pg/ml) \| 0 \| 31.2 \| 62.5 \| 125 \| 250 \| 500 \| 1000 \| 2000 O.D. \| 0.024 \| 0.152 \| 0.244 \| 0.463 \| 0.746 \| 1.212 \| 1.795 \| 2.321
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 229 \| 1188 \| 4782 \| 314 \| 1249 \| 4914 Standard deviation \| 12.14 \| 66.53 \| 277.36 \| 20.72 \| 86.18 \| 358.72 CV (%) \| 5.3% \| 5.6% \| 5.8% \| 6.6% \| 6.9% \| 7.3%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 229 \| 278 \| 272 \| 238 \| 254 \| 21.09 \| 8.3% Sample 2 \| 1188 \| 1214 \| 1275 \| 1228 \| 1226 \| 31.59 \| 2.5% Sample 3 \| 4782 \| 5075 \| 5245 \| 4924 \| 5006 \| 172.32 \| 3.4% *number of samples for each test n=16.
Capture antibody and Detection antibody	a monoclonal antibody from mouse\|a biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK2001.pdf

BOS-EK2001-datasheet.pdf

BOS-EK2001-safety-datasheet.pdf