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Human TNFRSF21/DR6 ELISA Kit PicoKine®

Human TNFRSF21/DR6 ELISA Kit PicoKine®

ArtNr	BOS-EK2004
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Neuroscience Aging & Neurological Disorders ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Tumor necrosis factor receptor superfamily member 21,Death receptor 6,CD358,TNFRSF21,DR6,UNQ437,PRO868
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	TNFRSF21
Gene Full Name	TNF receptor superfamily member 21
Manufacturer - Specificity	Natural and recombinant human TNFRSF21
Background	Death receptor 6 (DR6), also known as tumor necrosis factor receptor superfamily member 21 (TNFRSF21), is a cell surface receptor of the tumor necrosis factor receptor superfamily which activates the JNK and NF-κB pathways. This gene is mapped to 6p12.3. This gene encodes a member of the tumor necrosis factor receptor superfamily. The encoded protein activates nuclear factor kappa-B and mitogen-activated protein kinase 8 (also called c-Jun N-terminal kinase 1), and induces cell apoptosis. Through its death domain, the encoded receptor interacts with tumor necrosis factor receptor type 1-associated death domain (TRADD) protein, which is known to mediate signal transduction of tumor necrosis factor receptors. Knockout studies in mice suggest that this gene plays a role in T-helper cell activation, and may be involved in inflammation and immune regulation.
Immunogen	Expression system for standard: NS0; Immunogen sequence: Q42-H349
Protein Name	Tumor necrosis factor receptor superfamily member 21
Protein Function	Promotes apoptosis, possibly via a pathway that involves the activation of NF-kappa-B. Can also promote apoptosis mediated by BAX and by the release of cytochrome c from the mitochondria into the cytoplasm. Plays a role in neuronal apoptosis, including apoptosis in response to amyloid peptides derived from APP, and is required for both normal cell body death and axonal pruning. Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N-terminal fragment of APP (N-APP). N-APP binds TNFRSF21; this triggers caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). Negatively regulates oligodendrocyte survival, maturation and myelination. Plays a role in signaling cascades triggered by stimulation of T-cell receptors, in the adaptive immune response and in the regulation of T-cell differentiation and proliferation. Negatively regulates T-cell responses and the release of cytokines such as IL4, IL5, IL10, IL13 and IFNG by Th2 cells. Negatively regulates the production of IgG, IgM and IgM in response to antigens. May inhibit the activation of JNK in response to T-cell stimulation.
Subcellular Localization	Cell membrane. Single-pass type I membrane protein.
Description	Human TNFRSF21/DR6 ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF21 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Sample Data	Concentration (pg/ml) \| 0 \| 15.6 \| 31.2 \| 62.5 \| 125 \| 250 \| 500 \| 1000 O.D. \| 0.033 \| 0.081 \| 0.130 \| 0.220 \| 0.408 \| 0.793 \| 1.392 \| 2.219
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 21 \| 94 \| 378 \| 26 \| 103 \| 431 Standard deviation \| 0.86 \| 5.83 \| 17.01 \| 1.22 \| 6.59 \| 23.27 CV (%) \| 4.1% \| 6.2% \| 4.5% \| 4.7% \| 6.4% \| 5.4%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 21 \| 22 \| 25 \| 23 \| 22 \| 1.47 \| 6.6% Sample 2 \| 94 \| 91 \| 107 \| 99 \| 97 \| 6.05 \| 6.2% Sample 3 \| 378 \| 402 \| 415 \| 388 \| 395 \| 14.00 \| 3.5% *number of samples for each test n=16.
Capture antibody and Detection antibody	a monoclonal antibody from mouse\|a biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin, EDTA or citrate
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK2004.pdf

BOS-EK2004-datasheet.pdf

BOS-EK2004-safety-datasheet.pdf