Mouse Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine®

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

GC, vitamin D binding protein

Vitamin D-binding protein (DBP), also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene. It is mapped to 5 E1; 5 44.32 cM. The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.

Mouse Vitamin DBP ELISA Kit PicoKine® (96 Tests). Quantitate Mouse GC in cell culture supernatants, cell lysates, serum, plasma (heparin) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

cell culture supernatants, cell lysates, serum, plasma (heparin) and urine.

Intra-Assay Precision | Inter-Assay Precision
Sample | 1 | 2 | 3 | 1 | 2 | 3
n | 16 | 16 | 16 | 24 | 24 | 24
Mean (pg/ml) | 551 | 2528 | 8207 | 568 | 2328 | 8686
Standard deviation | 36.91 | 156.73 | 648.35 | 47.14 | 144.33 | 773.05
CV (%) | 6.7% | 6.2% | 7.9% | 8.3% | 6.2% | 8.9%

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

200 ng/tube

Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.