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Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine® Europäischer Partner

ArtNr BOS-EK2068
Hersteller Boster
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
Sensitivity <50 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Cadherin-12,Cdh12
Lieferbar
Manufacturer - Category
PicoKine® ELISA Kits
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name
Cdh12
Gene Full Name
cadherin 12
Manufacturer - Specificity
Natural and recombinant mouse Cadherin-12
Background
Cadherin-12 is a protein that in humans is encoded by the CDH12 gene. It is mapped to 15 A2; 15 8.25 cM. This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. This gene is expressed in both the excitatory and inhibitory neurons in various areas of the adult central nervous system including the cerebellum, neocortex, hippocampus and in different subcortical nuclei. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 15.
Immunogen
Expression system for standard: NS0; Immunogen sequence: L18-A716
Protein Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Subcellular Localization
Cell membrane. Single-pass type I membrane protein.
Description
Mouse BR-Cadherin-12 Cdh12 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cdh12 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type
cell culture supernatants, serum and plasma (heparin, EDTA).
Sample Data
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000
O.D. | 0.038 | 0.147 | 0.243 | 0.407 | 0.803 | 1.145 | 1.914 | 2.250
Intra Assay Consistency & Inter Assay Consistency
Intra-Assay Precision | Inter-Assay Precision
Sample | 1 | 2 | 3 | 1 | 2 | 3
n | 16 | 16 | 16 | 24 | 24 | 24
Mean (pg/ml) | 169 | 933 | 2976 | 183 | 1016 | 4238
Standard deviation | 7.10 | 40.12 | 122.02 | 12.08 | 73.15 | 322.09
CV (%) | 4.2% | 4.3% | 4.1% | 6.6% | 7.2% | 7.6%
Reproducibility
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 169 | 180 | 186 | 171 | 176 | 6.87 | 3.9%
Sample 2 | 933 | 882 | 941 | 864 | 905 | 32.74 | 3.6%
Sample 3 | 2976 | 3195 | 3034 | 3038 | 3060 | 81.29 | 2.6%
*number of samples for each test n=16.
Capture antibody and Detection antibody
a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Kit Components
Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4
Standard
10 ng/tube
Anticoagulant
heparin or EDTA
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Manufacturer - Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
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