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Mouse CD99L2 ELISA Kit PicoKine®

Mouse CD99L2 ELISA Kit PicoKine®

ArtNr	BOS-EK2091
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Oncology ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Immunostaining Bone Cancer
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	<50 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CD99 antigen-like protein 2,MIC2-like protein 1,CD99,Cd99l2,Mic2l1
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	Cd99l2
Gene Full Name	CD99 antigen-like 2
Manufacturer - Specificity	Natural and recombinant mouse CD99L2
Background	CD99 antigen-like protein 2 is a protein that in humans is encoded by the CD99L2 gene. It is mapped to X; X A7.3. This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: D26-G161
Protein Function	Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation.
Subcellular Localization	Cell membrane. Single-pass type I membrane protein. Extracellular side. Cell junction.
Description	Mouse CD99L2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd99l2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Highly expressed in the nervous system, including brain, dentate nucleus of hippocampus, granular and Purkinje cells of cerebellum, brain stem nucleus and choroid plexus. Expressed in peripheral blood T- and B-cells and neutrophils. Almost undetectable in bone marrow-derived neutrophils. Also expressed in thymocytes with higher expression in cortical thymocytes than in medullary thymocytes. Expressed at high levels in testis (mostly in germ cells and Sertoli cells) and ovary (mostly in granulosa cells). Expressed in lung, heart, kidney and liver; however, expression in heart, kidney and liver seems restricted to endothelial cells. Highly expressed in endothelial cells and to a lower level in vascular smooth muscle cells. Low expression in spleen.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Sample Data	Concentration (pg/ml) \| 0 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 \| 20000 O.D. \| 0.062 \| 0.144 \| 0.218 \| 0.378 \| 0.612 \| 0.939 \| 1.446 \| 2.059
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 467 \| 1310 \| 7832 \| 529 \| 2102 \| 8406 Standard deviation \| 19.15 \| 56.33 \| 360.27 \| 30.68 \| 145.04 \| 605.23 CV (%) \| 4.1% \| 4.3% \| 4.6% \| 5.8% \| 6.9% \| 7.2%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 467 \| 427 \| 448 \| 472 \| 453 \| 17.72 \| 3.9% Sample 2 \| 1310 \| 1396 \| 1545 \| 1357 \| 1402 \| 87.99 \| 6.2% Sample 3 \| 7832 \| 7176 \| 7216 \| 8274 \| 7624 \| 456.32 \| 5.9% *number of samples for each test n=16.
Capture antibody and Detection antibody	a monoclonal antibody from rat\|a biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	20 ng/tube
Anticoagulant	heparin, EDTA or citrate
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK2091.pdf

BOS-EK2091-datasheet.pdf

BOS-EK2091-safety-datasheet.pdf