CD71/TfR Colorimetric Cell-Based ELISA Kit

Store at 4°C for up to 6 months.

TFRC

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).

The CD71/TfR Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CD71/TfR protein expression profile in cells. The kit can be used for measuring the relative amounts of CD71/TfR in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CD71/TfR.