GSK3alpha/beta (Phospho-Tyr279/216) Colorimetric Cell-Based ELISA Kit

Cell-Based ELISA Kits

50981 MW

Glycogen synthase kinase-3 alpha

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation.

The GSK3alpha/beta (Phospho-Tyr279/216) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor GSK3alpha/beta (Phospho-Tyr279/216) protein expression profile in cells. The kit can be used for measuring the relative amounts of GSK3alpha/beta (Phospho-Tyr279/216) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on GSK3alpha/beta (Phospho-Tyr279/216).