AMPK beta1 (Phospho-Ser181) Colorimetric Cell-Based ELISA Kit

Cell-Based ELISA Kits

30382 MW

5'-AMP-activated protein kinase subunit beta-1

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

The AMPK beta1 (Phospho-Ser181) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AMPK beta1 (Phospho-Ser181) protein expression profile in cells. The kit can be used for measuring the relative amounts of AMPK beta1 (Phospho-Ser181) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on AMPK beta1 (Phospho-Ser181).