PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Colorimetric Cell-Based ELISA Kit

Store at 4°C for up to 6 months.

PIK3R1

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).

The PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) protein expression profile in cells. The kit can be used for measuring the relative amounts of PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199).