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Human PCSK9 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human PCSK9 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

ArtNr	BOS-EZ1147
Hersteller	Boster
Menge	5 plates/kit
Kategorie	Neuroscience Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience Obesity ELISA Lipoprotein Receptors & Associated Proteins ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Convertase subtilisin/kexin type 9 preproprotein,FH3,HCHOLA3,Hypercholesterolemia autosomal dominant 3,LDLCQ1,NARC 1,NARC-1,NARC1,Neural apoptosis regulated convertase 1,Neural apoptosis-regulated convertase 1,PC 9,PC9,PCSK 9,PCSK9,PCSK9_HUMAN,Proprotein convertase 9,Proprotein convertase PC9,Proprotein convertase subtilisin/kexin type 9,PSEC0052,Subtilisin/kexin like protease PC9,Subtilisin/kexin-like protease PC9
Lieferbar
Manufacturer - Category	EZ-Set™ ELISA Kits (Ab Pairs)
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	PCSK9
Gene Full Name	proprotein convertase subtilisin/kexin type 9
Manufacturer - Specificity	Natural and recombinant human PCSK9
Background	Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins (LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.
Immunogen	Expression system for standard: NS0; Immunogen sequence: S153-Q692
Manufacturer - Research Category	Amino Acid Metabolism, Amino Acids, Cancer, Cardiovascular, Cell Biology, Cholesterol Metabolism, Intracellular, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neural Stem Cells, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Stem Cells
Protein Function	Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na+ channel (ENaC)-mediated Na+ absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
Subcellular Localization	Endoplasmic reticulum, Golgi apparatus, Secreted, Lysosome, Endosome, Cytoplasm, Cell surface
Description	Human PCSK9 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PCSK9 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Tissue Specificity	Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Sample Type	cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).
Sample Data	Concentration (pg/ml) \| 0 \| 156 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 O.D. \| 0.058 \| 0.112 \| 0.14 \| 0.235 \| 0.391 \| 0.73 \| 1.314 \| 2.215
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 229 \| 1991 \| 5404 \| 215 \| 1927 \| 5944 Standard deviation \| 16.03 \| 125.43 \| 243.18 \| 17.63 \| 132.96 \| 285.31 CV (%) \| 7% \| 6.3% \| 4.5% \| 8.2% \| 6.9% \| 4.8%
Kit Components	Catalog number \| Description \| Quantity \| Storage of opened/reconstituted material EZ1147-CA \| Rabbit anti- human PCSK9 polyclonal antibody (Capture Antibody) \| 500 µ l, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1147-DA \| Biotinylated goat anti- human PCSK9 polyclonal antibody (Detection Antibody) \| 500 µ l, 28 µ g/mL (recommended dilution 1:100) AR1103 \| Avidin-Biotin-Peroxidase Complex (ABC) \| 500 µ l (recommended dilution 1:100) EK1147-ST \| Lyophilized recombinant human PCSK9 standard \| 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.
Anticoagulant	heparin, EDTA or citrate
Material Required But Not Provided	1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T). Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Manufacturer - Alias	Convertase subtilisin/kexin type 9 preproprotein, FH3, HCHOLA3, Hypercholesterolemia autosomal dominant 3, LDLCQ1, NARC 1, NARC-1, NARC1, Neural apoptosis regulated convertase 1, Neural apoptosis-regulated convertase 1, PC 9, PC9, PCSK 9, PCSK9, PCSK9_HUMAN, Proprotein convertase 9, Proprotein convertase PC9, Proprotein convertase subtilisin/kexin type 9, PSEC0052, Subtilisin/kexin like protease PC9, Subtilisin/kexin-like protease PC9
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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