Human FABP4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

EZ-Set™ ELISA Kits (Ab Pairs)

FABP4

Natural and recombinant human FABP4

Expression system for standard: E.coli; Immunogen sequence: M1-A132

Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.

Human FABP4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FABP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.

Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000
O.D. | 0.044 | 0.153 | 0.19 | 0.309 | 0.497 | 1.002 | 1.482 | 2.157

Catalog number | Description | Quantity | Storage of opened/reconstituted material
EZ1459-CA | Rabbit anti- human FABP4 polyclonal antibody (Capture Antibody) | 500 µ l, 0.4 mg/mL
(recommended dilution 1:100)
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ1459-DA | Biotinylated goat anti- human FABP4 polyclonal antibody (Detection Antibody) | 500 µ l, 6 µ g/mL
(recommended dilution 1:100)
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µ l (recommended dilution 1:100)
EK1459-ST | Lyophilized recombinant human FABP4 standard | 10 ng/tubex3
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit.

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)