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Human PDGF-AB PicoKine® Quick ELISA Kit

Human PDGF-AB PicoKine® Quick ELISA Kit

ArtNr	BOS-FEK0484
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Oncology By Cell Type Glioma Hematology Lymphoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<2 pg/ml
Sequence	Belongs to the PDGF/VEGF growth factor family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Platelet-derived growth factor subunit B,PDGF subunit B,PDGF-2,Platelet-derived growth factor B chain,Platelet-derived growth factor beta polypeptide,Proto-oncogene c-Sis,Becaplermin,PDGFB,PDGF2,SIS
Lieferbar
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	27283 MW
Gene Name	PDGF-AB
Gene Full Name	Platelet-derived growth factor subunit B
Manufacturer - Specificity	Natural and recombinant human PDGF-AB
Background	The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27, 281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant human PDGF-AB with the molecular mass of 27KDa.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: (S87-T211) + (S82-T190)
Protein Name	Platelet-derived growth factor subunit B, Platelet-derived growth factor subunit A
Protein Function	Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
Subcellular Localization	Secreted. Released by platelets upon wounding.
Description	Human PDGF-AB PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human PDGF-AB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sequence Similarities	Belongs to the PDGF/VEGF growth factor family.
Sample Data	Concentration (pg/ml) \| 0 \| 31.25 \| 62.5 \| 125 \| 250 \| 500 \| 1000 \| 2000 O.D. \| 0.023 \| 0.126 \| 0.252 \| 0.423 \| 0.922 \| 1.358 \| 1.856 \| 2.095
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 60 \| 259 \| 808 \| 62 \| 271 \| 865 Standard deviation \| 3.96 \| 14.76 \| 51.71 \| 4.52 \| 19.51 \| 68.33 CV (%) \| 6.6% \| 5.7% \| 6.4% \| 7.3% \| 7.2% \| 7.9%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 60 \| 69 \| 60 \| 60 \| 62 \| 3.89 \| 6.2% Sample 2 \| 259 \| 267 \| 273 \| 260 \| 264 \| 5.67 \| 2.1% Sample 3 \| 808 \| 809 \| 832 \| 839 \| 822 \| 13.72 \| 1.6% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity 96-well plate\|1 lyophilized Standard\| Biotinylated detection antibody (50x)\|100 ul Avidin-Biotin-Peroxidase Complex (30x)\|400 µl Sample diluent\|30 ml Antibody diluent\|12 ml Avidin-Biotin-Peroxidase Diluent\|12 ml Wash Buffer (25x)\|20 ml Color Developing Reagent (TMB)\|10 ml Stop Solution\|10 ml Plate Sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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