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Mouse sL-Selectin PicoKine® Quick ELISA Kit Europäischer Partner

ArtNr BOS-FEK0504
Hersteller Boster
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
Sensitivity <5 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias L-selectin ;Sell ;
Lieferbar
Manufacturer - Category
Fast ELISA Kits
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight
38463 MW
Manufacturer - Gene Name
Sell
Gene Full Name
selectin L
Sample Type
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sample Data
Concentration (pg/ml)
Manufacturer - Specificity
Natural and recombinant mouse sL-Selectin
Intra Assay Consistency & Inter Assay Consistency
Intra-Assay PrecisionInter-Assay Precision
Sample
Reproducibility
LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1
Capture antibody and Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
Kit Components
Description|Quantity
96-well plate|1
lyophilized Standard|
Biotinylated detection antibody (50x)|100 ul
Avidin-Biotin-Peroxidase Complex (30x)|400 µl
Sample diluent|30 ml
Antibody diluent|12 ml
Avidin-Biotin-Peroxidase Diluent|12 ml
Wash Buffer (25x)|20 ml
Color Developing Reagent (TMB)|10 ml
Stop Solution|10 ml
Plate Sealers|4
Standard
10 ng/tube
Anticoagulant
heparin or EDTA
Background
L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
Immunogen
Expression system for standard: NS0; Immunogen sequence: W39-N332
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Protein Name
L-selectin
Description
Mouse sL-Selectin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Sell in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
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