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Mouse MCP-1 PicoKine® Quick ELISA Kit

Mouse MCP-1 PicoKine® Quick ELISA Kit

ArtNr	BOS-FEK0568
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Neuroscience Neuroinflammation ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	<6 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	C-C motif chemokine 2,Monocyte chemoattractant protein 1,Monocyte chemotactic protein 1,MCP-1,Platelet-derived growth factor-inducible protein JE,Small-inducible cytokine A2,Ccl2,Je,Mcp1,Scya2
Lieferbar
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Calculated Molecular weight	16326 MW
Gene Name	CCL2
Gene Full Name	C-C motif chemokine 2
Manufacturer - Specificity	Natural and recombinant mouse MCP-1
Background	Monocyte chemotactic protein-1 (MCP-1) is a member of the small inducible gene (SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell expressed and secreted, and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1. The standard used in this kit is recombinant mouse MCP-1 (Q24-R96) with the molecular mass of 8.5Kda.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: Q24-R96
Protein Name	C-C motif chemokine 2
Description	Mouse MCP-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Ccl2 in cell culture supernatants and serum. Sensitivity: 6pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants and serum.
Sample Data	Concentration (pg/ml) \| 0 \| 15.6 \| 31.2 \| 62.5 \| 125 \| 250 \| 500 \| 1000 O.D. \| 0.014 \| 0.051 \| 0.090 \| 0.193 \| 0.406 \| 0.726 \| 1.465 \| 1.897
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 39 \| 224 \| 394 \| 40 \| 231 \| 398 Standard deviation \| 1.56 \| 10.75 \| 27.18 \| 2 \| 12.47 \| 27.46 CV (%) \| 4% \| 4.8% \| 6.9% \| 5% \| 5.4% \| 6.9%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 39 \| 36 \| 34 \| 34 \| 35 \| 2.04 \| 5.8% Sample 2 \| 224 \| 228 \| 228 \| 219 \| 224 \| 3.69 \| 1.6% Sample 3 \| 394 \| 375 \| 431 \| 394 \| 398 \| 20.3 \| 5.1% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from rat\|polyclonal antibody from goat
Kit Components	Description\|Quantity\|Volume Anti-tag Pre-coated 96-well Strip Microplate\|1\|12 strips of 8 wells Standard\|\|2 Antibody A\|1\|3ml Antibody B\|1\|3ml Sample Diluent\|1\|15ml TBS-T Wash Buffer (25x)\|1\|12ml Color Developing Reagent (TMB)\|1\|10ml Stop Solution\|1\|10ml Adhesive Plate Sealers\|2\|Piece
Standard	10 ng/tube
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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BOS-FEK0568-datasheet.pdf

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