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Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit

Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit

ArtNr	BOS-FEK0904
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Oncology By Cell Type Carcinoma Hematology Leukemia ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Testicular & Prostate Cancer Gallbladder Cancer Gastric Cancer Pancreatic Cancer Thyroid Cancer Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
Sequence	Belongs to the immunoglobulin superfamily. CEA family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Carcinoembryonic antigen-related cell adhesion molecule 5,Carcinoembryonic antigen,CEA,Meconium antigen 100,CD66e,CEACAM5,CEA
Lieferbar
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	76795 MW
Gene Name	CEACAM5
Gene Full Name	Carcinoembryonic antigen-related cell adhesion molecule 5
Manufacturer - Specificity	Natural and recombinant human CEA
Background	Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180, 000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
Immunogen	Expression system for standard: NS0; Immunogen sequence: K35-A685
Protein Name	Carcinoembryonic antigen-related cell adhesion molecule 5
Protein Function	Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
Subcellular Localization	Cell membrane; Lipid-anchor, GPI-anchor.
Description	Human CEA / Carcino Embryonic Antigen PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CEACAM5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sequence Similarities	Belongs to the immunoglobulin superfamily. CEA family.
Sample Data	Concentration (pg/ml) \| 0 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 \| 20000 O.D. \| 0.011 \| 0.056 \| 0.111 \| 0.240 \| 0.381 \| 0.713 \| 1.296 \| 1.967
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 776 \| 1668 \| 10176 \| 724 \| 1662 \| 10501 Standard deviation \| 39.57 \| 124 \| 407.04 \| 39.09 \| 137.94 \| 420.04 CV (%) \| 4% \| 7.5% \| 4% \| 5.4% \| 8.3% \| 4%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 776 \| 816 \| 775 \| 762 \| 782 \| 20.25 \| 2.5% Sample 2 \| 1668 \| 1752 \| 1723 \| 1646 \| 1697 \| 42.25 \| 2.4% Sample 3 \| 10176 \| 9323 \| 9418 \| 9597 \| 9628 \| 331.05 \| 3.4% *number of samples for each test n=16.
Capture antibody and Detection antibody	polyclonal antibody from rabbit\|polyclonal antibody from rabbit
Kit Components	Description\|Quantity 96-well plate\|1 lyophilized Standard\| Biotinylated detection antibody (50x)\|100 ul Avidin-Biotin-Peroxidase Complex (30x)\|400 µl Sample diluent\|30 ml Antibody diluent\|12 ml Avidin-Biotin-Peroxidase Diluent\|12 ml Wash Buffer (25x)\|20 ml Color Developing Reagent (TMB)\|10 ml Stop Solution\|10 ml Plate Sealers\|4
Standard	20 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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BOS-FEK0904-datasheet.pdf

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