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Mouse SAP/PTX2/APCS PicoKine® Quick ELISA Kit Europäischer Partner

ArtNr BOS-FEK1208
Hersteller Boster
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
Sensitivity <50 pg/ml
Sequence Belongs to the pentaxin family.
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Serum amyloid P-component,SAP,Apcs,Ptx2,Sap
Lieferbar
Manufacturer - Category
Quick ELISA Kits
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight
26247 MW
Gene Name
Apcs
Gene Full Name
Serum amyloid P-component
Manufacturer - Specificity
Natural and recombinant mouse SAP
Background
Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called amyloid. It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q21-D224
Protein Name
Serum amyloid P-component
Subcellular Localization
Secreted.
Description
Mouse SAP/PTX2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Apcs in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sequence Similarities
Belongs to the pentaxin family.
Sample Data
Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100
O.D. | 0.002 | 0.065 | 0.127 | 0.246 | 0.436 | 0.787 | 1.246 | 1.831
Intra Assay Consistency & Inter Assay Consistency
Intra-Assay Precision | Inter-Assay Precision
Sample | 1 | 2 | 3 | 1 | 2 | 3
n | 16 | 16 | 16 | 24 | 24 | 24
Mean (pg/ml) | 3608 | 12778 | 47613 | 3726 | 13449 | 43062
Standard deviation | 144.32 | 715.56 | 2809.16 | 176.12 | 981.77 | 2626.78
CV (%) | 4% | 5.6% | 5.9% | 4.7% | 7.3% | 6.1%
Reproducibility
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 3608 | 4012 | 4067 | 4138 | 3956 | 205.96 | 5.2%
Sample 2 | 12778 | 11342 | 12753 | 12065 | 12234 | 589.38 | 4.8%
Sample 3 | 47613 | 50001 | 47706 | 49348 | 48667 | 1034.13 | 2.1%
*number of samples for each test n=16.
Capture antibody and Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
Kit Components
Description|Quantity
96-well plate|1
lyophilized Standard|
Biotinylated detection antibody (50x)|100 ul
Avidin-Biotin-Peroxidase Complex (30x)|400 µl
Sample diluent|30 ml
Antibody diluent|12 ml
Avidin-Biotin-Peroxidase Diluent|12 ml
Wash Buffer (25x)|20 ml
Color Developing Reagent (TMB)|10 ml
Stop Solution|10 ml
Plate Sealers|4
Standard
100 ng/tube
Anticoagulant
heparin or EDTA
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Manufacturer - Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
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