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Rat MIP-1Alpha/CCL3 ELISA Kit PicoKine®

Rat MIP-1Alpha/CCL3 ELISA Kit PicoKine®

ArtNr

BOS-EK1219

Hersteller

Boster

Menge

96 wells/kit, with removable strips.

Kategorie

Typ

Elisa-Kit

Applikationen

ELISA

Specific against

Rat (Rattus norvegicus)

Sensitivity

<1 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

C-C motif chemokine 3;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;Small-inducible cytokine A3;Ccl3;Mip1a, Scya3;

Lieferbar

Manufacturer - Category

PicoKine® ELISA Kits

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular weight

10335 MW

Manufacturer - Gene Name

CCL3

Gene Full Name

C-C motif chemokine 3

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Sample Data

Concentration (pg/ml)

Manufacturer - Specificity

Natural and recombinant rat MIP-1 alpha

Intra Assay Consistency & Inter Assay Consistency

Intra-Assay Precision

Inter-Assay Precision

Sample

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin, EDTA or Citrate
Background	Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7, 889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: A24-A92
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Research Category	Antiviral Signaling, Chemokines, Immune System Diseases, Immunology, Innate Immunity, Macrophage/Inflammation
Protein Name	C-C motif chemokine 3
Protein Function	Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.
Subcellular Localization	Secreted.
Description	Rat MIP-1Alpha/CCL3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK1219.pdf

BOS-EK1219-datasheet.pdf

BOS-EK1219-safety-datasheet.pdf