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Rat CCL7/MCP-3 ELISA Kit PicoKine® Europäischer Partner

ArtNr BOS-EK1300
Hersteller Boster
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Rat (Rattus norvegicus)
Sensitivity <10 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias C-C motif chemokine 7; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; MCP-3; Small-inducible cytokine A7; Ccl7; Mcp3, Scya7;
Lieferbar
Manufacturer - Category
PicoKine® ELISA Kits
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Manufacturer - Gene Name
Ccl7
Gene Full Name
C-C motif chemokine ligand 7
Sample Type
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sample Data
Concentration (pg/ml)
Manufacturer - Specificity
Natural and recombinant rat CCL7
Intra Assay Consistency & Inter Assay Consistency
Intra-Assay PrecisionInter-Assay Precision
Sample
Reproducibility
LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1
Capture antibody and Detection antibody
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Kit Components
Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4
Standard
10 ng/tube
Anticoagulant
heparin or EDTA
Background
Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
Immunogen
Expression system for standard: E.coli; Immunogen sequence: Q24-P97
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Manufacturer - Research Category
Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation
Protein Function
Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity (By similarity).
Subcellular Localization
Secreted.
Description
Rat CCL7/MCP-3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Ccl7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
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