MSH6 MutS Homolog 6 Human Recombinant Protein

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Hersteller Boster
Kategorie
Typ Proteins Recombinant
Specific against other
Format Sterile Filtered clear solution.
Menge 5ug
ArtNr BOS-PROTP52701
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Gene name
MSH6
Conjugate
No
Contents
MSH6 is supplied in 50mM Tris-HCl, pH-7.5, 10mM L-glutathione (reduced).
Description
MSH6 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 51 amino acids (350-400 a.a.) having a total Mw of 33 kDa. Human MSH6 is fused to a GST tag and purified by proprietary chromatographic techniques.
Short description
MSH6 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 533 kDa. Human MSH6 is fused to a GST tag and purified by proprietary chromatographic techniques.
Uniprot ID
P52701
Storage
Store in -20° C for long term storage. After reconstitution, store in 4° C for short term usage within a few days. Avoid freeze-thaw cycles.
Synonyms
DNA mismatch repair protein Msh6
Protein function
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
Subcellular localization
Nucleus
Source
E. Coli
Shipping
Shipped with wet ice

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Menge: 5ug
Lieferbar: In stock
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